Coding

Part:BBa_K3206003:Design

Designed by: Matthew Rogan   Group: iGEM19_Newcastle   (2019-10-15)


PepD gene


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 207
    Illegal AgeI site found at 1360
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This part was made compatible with type IIs assembly.


Source

Escherichia coli K12, part was sourced from UniProt https://www.uniprot.org/uniprot/P15288

References

Henrich, B, Monnerjahn, U & Plapp, R, 1990. Peptidase D gene (pepD) of Escherichia coli K-12: nucleotide sequence, transcript mapping, and comparison with other peptidase genes. The Journal of Bacteriology, 172(8), pp.4641–4651.